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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Lab Invest. 2008 Sep 8;88(11):1178–1191. doi: 10.1038/labinvest.2008.83

Figure 4.

Figure 4

Chemokine mRNA and protein in the liver over 0–48 hours after a single injection of ricin (40 μg/kg) in C57BL/6 mice. A,C,E. Messenger RNA for CXCL1/KC, CXCL2/MIP-2, and CCL2/MCP-1(JE) is shown as a ratio of specific message/value of a house-keeping gene (18S or L32), determined by Northern blotting (CXCL1/KC) or by ribonuclease protection assay (CXCL2/MIP-2, CCL2/MCP-1). Specific mRNA for these chemokines was not detected at 0 hours. Two mice were analyzed per time point. B,D,E. Chemokine protein in liver homogenates after ricin challenge, quantified by paired antibody enzyme-linked immuno-absorbent assay, described in Materials and Methods. Shown is the mean +/− 1 SD. Homogenates were analyzed in duplicate from each of two mice per time point (14 mice total per cytokine). *, p< 0.05, comparing values at the indicted hours with that at 0 hours.