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Activation of PKA at the leading edge is integrin dependent. (A) CHO-α4 cell monolayers adhered on CS1 and expressing pmAKAR3 were wounded, and migration was allowed to proceed in the presence of 2 μg/ml HP2/1 (anti-α4) or PB1 (anti-α5) monoclonal antibodies as indicated. (B) CHO cell monolayers adhered on fibronectin and expressing pmAKAR3 were wounded, and migration was allowed to proceed in the presence of 2 μg/ml HP2/1 or PB1 monoclonal antibodies as indicated.
