Figure 7.
Virus replication and folding of the non–β-branched fill-gap mutants FG1 (C385A/C418L/T415I) and FG2 (C385L/C418A/T415I). (A) Sequences of the V4 loop and flanking regions of wt, mutant, and revertant viruses. The original mutations are indicated with gray circles, the mutations and reversions with black letters. (B) SupT1 T-cells (50 × 103) were infected with 2.5 ng CA-p24, and viral spread was measured for 14 d. Inset, Gp120 and CA-p24 contents in virus were measured by ELISA. The gp120 amounts were standardized for CA-p24 input and the gp120 contents of mutants in the respective fractions are given as percentages of the wt gp120 contents (arbitrarily set at 1).