Figure 2.

The N-terminal region and the C-terminal Rho-GAP domain are both required for LRG1's cellular function. (A) Genomic organization of lrg-1 locus. The four exons are represented in white, the three introns in black. The lrg-1 gene encodes for a protein with three LIM domains (brown) in the N-terminal half and one Rho-GAP domain (blue) in the C-terminal part of the protein. Green arrows represent expression constructs under the control of the endogenous promoter. (B) Complementation experiments of lrg-1(12-20) transformed with the indicated constructs. Transformants were grown at 37°C to determine the complementation capability of the respective construct. Bar, 200 μm.