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. 2008 Nov;19(11):4554–4569. doi: 10.1091/mbc.E07-12-1266

Figure 3.

Figure 3.

LRG1 localization to sites of growth at the hyphal tip and the septum is dependent on functional LIM domains. (A) MYC::LRG1 and MYC::LRG11–847 immunolocalized in vesicular-reticulate structures that are enriched at hyphal tips, whereas LRG1781–1279::MYC mis-localized to the hyphal cortex. Bar, 10 μm. (B) Localization data generated in the ApoTome modus indicate an apical vesicular–reticulate localization of GFP::LRG1 and a more diffuse localization of GFP::LRG1*. Bar, 5 μm. (C) GFP::LRG1 localized as a crescent at growing hyphal tips and at septae (left), whereas GFP::LRG1* containing the three mutated LIM domains did localize in a diffuse manner throughout the cell (right). Bar, 5 μm.