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. 2008 Nov;19(11):4968–4979. doi: 10.1091/mbc.E08-03-0259

Figure 5.

Figure 5.

Nodal and ALK7-ca up-regulate cyclin G2 and down-regulate Skp1/2 expression. (A) ALK7-ca increased cyclin G2 level. OV2008 cells were transiently transfected with CCNG2-V5 and ALK7-ca, alone or in combination, for 16 h and then recovered for 0–24 h. Cotransfection with ALK7-ca resulted in an increase in cyclin G2 level. (B) ALK7 prevented cyclin G2 degradation. OV2008 cells were transfected with CCNG2-V5 and ALK7-ca and then recovered in the presence of cycloheximide (CHX, 5 μg/ml) or DMSO (control) for 6 h. (C) Cotransfection of Nodal and CCNG2-V5 resulted in an increase in cyclin G2 level at 6 h after overnight transfection. (D and E) Effects of Nodal and ALK7-ca on Skp1 (D) and Skp2 (E) expression. OV2008 cells were transiently transfected with Nodal or ALK7-ca for 16 h. Total protein was extracted at different time points after transfection, subjected to SDS-PAGE and Western blotting, and probed by anti-Skp1 and -Skp2 antibodies. Histograms show normalized densitometry data (mean±SEM) from three experiments. *p<0.05 versus EV control at the same time point.