Figure 3.

Keratinocytes from Slug−/− mice fail to spread and migrate from skin explants, even in the presence of EGF. (A) Skin explants from a wild-type (wt) and a Slug-LacZ homozygote mouse (−/−) were cultured ex vivo with 10 ng/ml EGF. (B) Phase-contrast micrographs and keratin (CK) immunolocalization in cells migrating from wild-type and Slug−/− skin explants. Keratinocytes are identified by keratin reactivity. The results shown are representative of four separate experiments. Black arrow, mesenchymal cells negative for keratin immunolabeling; white arrow; direction of keratinocyte migration; broken white line, keratinocyte migrating front; *, skin explant. Scale bar, 25 μm.