Figure 5.
Altered gene expression profiles among fosB mutant and wild-type ES cells. (A) Expression of genes for basement membrane proteins. RNA prepared from quiescent ES cells or ES cells 4 h after serum stimulation were subjected to RT-PCR analysis. The relative amount of each RT-PCR product to that of the quiescent wild type was normalized with that of Gapdh mRNA and is shown in parentheses. (B) Expression of ΔFosB protein in fosBd/d ES cells. Nuclear extracts (200 μg per lane) prepared from exponentially growing or quiescent ES cells were subjected to Western blotting with anti-FosB(N; top panel). Membranes stained with CBB are shown (bottom panel). The arrowhead indicates p32/36, and the arrow indicates p24. (C). RT-PCR analysis of genes for basement membrane proteins in exponentially growing ES cells. The amount of each RT-PCR product relative to that of the wild type was normalized with that of Gapdh mRNA and is shown in parenthesis. (D) RT-PCR analysis of Tgfb1 and Tgfb1i1 expression. The amount of each RT-PCR product relative to that of the exponentially growing wild type (left panel, exponential) or to that of the quiescent wild type (right panel, quiescent and serum-stimulated) was normalized with that of Gapdh mRNA, and is shown in parentheses.