Figure 6.

A jamp-1 deleted C. elegans strain exhibits basal stress and is hypersensitive to ER stress. (A) Increased sensitivity to ER stress exposure of a jamp-1(ok765) mutant. N2 (wild-type) and jamp-1(ok765) embryos were treated with indicated concentrations of tunicamycin, and developmental stages were assessed after a 72-h incubation on a bacterial lawn at 20°C. Animals were categorized as follows: adults and L4, arrested L3 and younger, and dead larvae. Each category is represented by the bar graph as the percentage of the total number of embryos (indicated above each bar). (B) Elevated basal expression of the hsp-4::gfp transcriptional reporter in jamp-1(ok765) worms. GFP fluorescence of control WT larvae and jamp-1(ok765) mutant carrying the hsp-4::gfp reporter. Both WT and mutant worms were grown in normal medium at 20°C. Wild-type (N2) larvae (top) exhibit low GFP levels. Autofluorescent granules in the cytoplasm of intestinal cells are shown (arrow). The jamp-1(ok765) larvae (bottom) show elevated fluorescence in intestine and exhibit high-GFP expression in anterior (AI) and posterior (PI) intestinal cells (arrows).