Figure 1.

Screen for regulators of Swe1p degradation identifies Elm1p. (A) In the starting strain for the screen (DLY 6940), the only copy of MIH1 is carried on a plasmid that also confers a red colony color by virtue of the ADE3 gene (pDLB2064). Loss of the plasmid during colony growth yields sectored red and white colonies (left: white sectors are mih1Δ). However, if the strain acquires a mutation disabling Swe1p degradation (mutX), the mih1Δ cells will die, so white sectors will not develop and the colony will be uniformly red (right: illustrated for an hsl1 isolate). (B) Results of the screen.