Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov;19(11):4675–4686. doi: 10.1091/mbc.E08-06-0663

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 by The American Society for Cell Biology

PMC Copyright notice

Figure 5. — Pseudophosphorylation of Hsl1p T273 suppresses mih1Δ elm1Δ synthetic lethality. (A) A mih1Δ elm1Δ strain kept alive with a URA3-marked plasmid carrying ELM1 was transformed with plasmids expressing the indicated allele of Hsl1p. On 5-FOA media only cells that have lost the ELM1 plasmid can live. Strains: HSL1 (DLY9409), hsl1^T273A (DLY9406), HSL1^T273E (DLY9407), HSL1^M177V (DLY9410), and HSL1^Δ^987–1100 (DLY9691). Cells were spotted onto YEPD with and without 5-FOA. (B) elm1Δ elongated cell morphology is suppressed by Hsl1p^T273E. DIC images of representative cells from proliferating asynchronous cultures of elm1Δ (DLY7704), hsl1^T273A elm1Δ (DLY9820), and HSL1^T273E elm1Δ (DLY10077) strains. (C) The G2/M cell cycle delay in elm1Δ cells is suppressed by Hsl1p^T273E. Cell cycle profiles were determined as described in Figure 3D for the following strains: WT (DLY8113), elm1Δ (DLY9806), hsl1^T273A elm1Δ (DLY9820), and HSL1^T273E elm1Δ (DLY10077). (D) gin4Δ elongated bud morphology is not suppressed by Hsl1p^T273E, but is exacerbated by Hsl1p^T273A. DIC images of representative cells from proliferating asynchronous cultures of gin4Δ (DLY4410), hsl1^T273A gin4Δ (DLY10076), and HSL1^T273E gin4Δ (DLY11008), strains.