Figure 6.

Neither Hsl1p^T273E nor Hsl1p^Δ987–1100 suppress G2 delay caused by septin disassembly. (A) HSL1 CDC12 (DLY8113), HSL1-myc cdc12-6 (DLY8745), HSL1^Δ987–1100 CDC12 (DLY8684), HSL1^Δ987–1100 cdc12-6 (DLY8709), HSL1^T273E CDC12 (DLY9391), and HSL1^T273E cdc12-6 (DLY7395) cells were grown to exponential phase at 18°C and shifted to 37°C for 3 h before fixation and DIC microscopy. (B) Cells from the same strains as in A were shifted from 18°C to 37°C for a total of 3 h. After the first hour, cells were treated with nocodozole to increase the amount of Cdc28p/Clb2. Total cell lysates were separated by SDS-PAGE and Cdc28p Tyr19 phosphorylation was assessed by Western blotting. (C) Cells from the same strains as in A were shifted from 18°C to 37°C for 3 h and lysed by TCA precipitation, and Hsl1p-myc and Cdc11p were detected by Western blotting.