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. 2008 Nov;19(11):4675–4686. doi: 10.1091/mbc.E08-06-0663

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© 2008 by The American Society for Cell Biology

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Figure 7. — Overexpression of the Hsl1p C-terminal region perturbs septin organization. (A) Overexpression of GST-Hsl1p^987–1518 causes development of elongated buds. Cells expressing GAL1-GST (DLY9587), GAL1-GST-HSL1^987–1100 (DLY9863), and GAL1-GST-HSL1^987–1518 (DLY9583) were grown on galactose media to induce overexpression, fixed, and imaged by DIC microscopy. (B) Overexpression of GST-Hsl1p^987–1518 causes septin disorganization. Cells containing GFP-CDC3 and GAL1-GST (DLY10097) or GAL1-GST-HSL1^987–1518 (DLY11002) were imaged by DIC and fluorescence microscopy. (C) Overexpression of GST-Hsl1p^987–1518 causes more severe bud elongation and neck defects in hsl1Δ cells. DIC images of hsl1Δ (JMY3–58) and hsl1Δ GAL1-GST-HSL1^987–1518 (DLY9543) cells. Arrows indicate aberrant necks. (D) Western blot showing expression of GST-Hsl1p fragments in strains DLY10097, DLY10098, and DLY11002. Blots were probed with anti-GST and anti-Cdc11 (loading control) antibodies.