Table 1.
Yeast strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| DLY1 | abar1 | Richardson et al. (1989) |
| DLY4410 | α gin4::kanR | Gladfelter et al. (2003) |
| DLY5000 | abar1 HSL1–13myc:URA3 HSL7-HA:kanR | Theesfeld et al. (2003) |
| DLY5336 | abar1 HSL1–13myc:URA3 cdc12–6:LEU2 HSL7-HA:kanR | This study |
| DLY5390 | abar1 hsl1K110R-13myc:URA3 HSL7-HA:kanR | Theesfeld et al. (2003) |
| DLY5844 | ahsl1::URA3 HSL7-HA:kanR | This study |
| DLY6940 | α ade1 ade3 mih1::LEU2+ pDLB2064 (MIH1 ADE3) | This study |
| DLY7395a | a3xHSL1T273E-13myc:TRP1 cdc12–6:LEU2 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY7400 | aHSL1–13myc::TRP hsl1::URA3 HSL7-HA:kanRcdc12–6:LEU2 | This study |
| DLY7451 | aHSL1T273E-13myc::TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY7601 | a/α elm1::URA3/ELM1, gin4::kanR/GIN4, cla4::TRP1/CLA4 mih1::LEU2/MIH1 | This study |
| DLY7697 | a/α mih1::TRP1/MIH1 elm1::LEU2/ELM1 | This study |
| DLY7704 | α elm1::LEU2 | This study |
| DLY7841 | α hsl1T273A-13myc:TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY7980 | ahsl1T273A-13myc:TRP1 hsl1::URA3 mih1::LEU2 HSL7-HA:kanR | This study |
| DLY8113 | aHSL1–13myc:TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY8116 | ahsl1D239A-13myc:TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY8117 | ahsl1K110R-13myc:TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY8119 | ahsl1N244A-13myc:TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY8684 | aHSL1Δ987–1100-13myc::TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY8709 | aHSL1Δ987–1100-13myc::TRP1 cdc12–6:LEU2 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY8745 | aHSL1–13myc:TRP1 cdc12–6:LEU2 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY9391a | a3xHSL1T273E-13myc::TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY9406 | ahsl1T273A-13myc::TRP1 elm1::LEU2 mih1::LEU2+ pDLB2729 (ELM1) | This study |
| DLY9407 | aHSL1T273E-13myc::TRP1 elm1::LEU2 mih1::LEU2+ pDLB2729 (ELM1) | This study |
| DLY9409 | aHSL1–13myc:TRP1 elm1::LEU2 mih1::LEU2+ pDLB2729 (ELM1) | This study |
| DLY9410 | aelm1::LEU2 mih1::LEU2+ pDLB2729 (ELM1)+ pDLB2510 (HSL1M177V-13myc) | This study |
| DLY9543 | ahsl1::LEU2:GAL1-GST-HSL1987–1518-13myc:TRP1 hsl1::URA3 | This study |
| DLY9583 | ahsl1::LEU2:GAL1-GST-HSL1987–1518:URA3 HSL1–13myc:TRP1 | This study |
| DLY9587 | aGAL1-GST:TRP1 | This study |
| DLY9691 | aHSL1Δ987–1100-13myc:TRP1 elm1::LEU2 mih1::LEU2+ pDLB2729 (ELM1) | This study |
| DLY9803 | ahsl1K110R-13myc:TRP1 hsl1::URA3 elm1::LEU2 | This study |
| DLY9804 | aHSL1T273E-13myc:TRP1 hsl1::URA3 elm1::LEU2 | This study |
| DLY9806 | aHSL1–13myc:TRP1 hsl1::URA3 elm1::LEU2 | This study |
| DLY9820 | ahsl1T273A-13myc:TRP1 hsl1::URA3 elm1::LEU2 | This study |
| DLY9863 | aGAL1-GST-HSL1987–1100:TRP1 | This study |
| DLY9882 | abar1 hsl1::URA3 mih1::LEU2 HSL1–13myc:TRP1 | This study |
| DLY9883 | abar1 hsl1::URA3 mih1::LEU2 swe1::LEU2 HSL1–13myc:TRP1 | This study |
| DLY10072 | aHSL1T273E-13myc:TRP1 hsl1::URA3 HSL7-HA:kanR | This study |
| DLY10076 | ahsl1T273A-13myc:TRP1 gin4::kanRhsl1::URA3 | |
| DLY10077 | aHSL1T273E-13myc:TRP1 hsl1::URA3 elm1::LEU2 | This study |
| DLY10097 | aCDC3-GFP:URA3 GAL1-GST:TRP1 | This study |
| DLY10098 | aCDC3-GFP:URA3 GAL1-GST-HSL1987–1100:TRP1 | This study |
| DLY11002 | aCDC3-GFP:URA3 hsl1::LEU2:GAL1-GST-HSL1987–1518-13myc:TRP1 | This study |
| DLY11005 | α HSL1T273E-13myc:TRP hsl1::URA3 HSL7-HA:kanRmih1::LEU2 | This study |
| DLY11006 | α HSL1T273E-13myc:TRP hsl1::URA3 HSL7-HA:kanRmih1::LEU2 swe1::LEU2 | This study |
| DLY11007 | ahsl1T273A-13myc:TRP hsl1:URA3 HSL7-HA:kanRmih1::LEU2 swe1::LEU2 | This study |
| DLY11008 | aHSL1T273E-13myc:TRP1 hsl1::URA3 gin4::kanR | This study |
| JMY3–58 | α hsl1::URA3 | This study |
a These strains derive from a transformant that expressed more Hsl1p (threefold by quantitative Western blotting) than wild-type or other mutant transformants, suggesting that three copies of the plasmid had integrated at TRP1.