Figure 4.
Meiotic progression and chromosome V segregation in cdc7-asNDT80 cells with or without PP1. cdc7-asNDT80 (NH874::CUP1- NDT80) carrying one copy of chromosome V marked by tetR-GFP was sporulated in the absence or presence of 15 μM PP1. (A) Meiotic progression: cells were stained with DAPI, and 200 cells were counted at the indicated time points after transfer to sporulation medium to determine the percentage of binucleate and tetranucleate cells. The number shown is the average of two independent experiments. (B) Chromosome V segregation: binucleate cells were analyzed for the number of green dots/nucleus. For cdc7-asNDT80 − PP1, the 6-h time point was used and 40 cells were examined. For cdc7-asNDT80 + PP1, the 8-h time point was used and 376 cells were examined. Left, representative picture of reductional segregation taken from cdc7-asNDT80. Right, representative picture of equational segregation taken from cdc7-asNDT80 + PP1. DAPI is stained with blue, tetR-GFP is green.