Table 2.

Sporulation and spore viability of various mutants in the presence or absence of Cdc7 kinase activity

Relevant genotype	Function	% sporulationa		% spore viability (no. asci)b−PP1
		−PP1	+PP1c
cdc7-as		83.5	0.0	91.3
sml1Δ cdc7-asd	Inhibitor of ribonucleotide reductase	94.0	0.0	97.1
mec1Δ smlΔ cdc7-as	Intra-S checkpoint	85.5	0.0	37.1
rad9Δ cdc7-as	DNA damage checkpoint	84.5	0.0	43.2
mek1Δ cdc7-as	Meiotic recombination checkpoint	64.0	0.0	<1.0
rec104 cdc7-as	DSB formation	51.0	0.0	<1.0
red1 cdc7-as	Formation of axial elements	56.8	0.0	<1.0

^a Cells (200) were assayed by light microscopy.

^b A minimum of 26 asci was dissected for each diploid.

^c PP1 (15 μM) was added to liquid sporulation cultures.

^d sml1Δ is needed to suppress the lethality of mec1Δ.