Figure 3.

HURP is required for chromatin-dependent microtubule nucleation. (A) Spindle assembly around DNA beads. Mock, ΔHURP, and ΔHURP+xlHURP extracts were incubated with DNA beads and Cy3-labeled tubulin (red) and fixed at 45–60 min. DNA was stained with Hoechst 33342 (blue). Scale bar, 15 μm. (B) Quantitation of structures around 100 DNA bead clusters in three independent experiments. Error bars, SD. (C) Ran-aster and Ran-spindle assembly. Mock, ΔHURP, and ΔHURP+xlHURP extracts were incubated with RanQ69L and Cy3-labeled tubulin and fixed at the indicated time points. Scale bar, 15 μm.