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. 2008 Oct 17;5:26. doi: 10.1186/1743-7075-5-26

Table 1.

Research Design

Generation Breeding Dam Prenatal/Nursing Diet Offspring Post weaning Diet
F0 Low Protein Energy-Restricted
F1 Energy-Restricted Energy-Restricted
F2 Energy-Restricted Energy-Restricted
F3 Energy-Restricted F3.1 Energy-Restricted
F3.2 Nutritionally-Adequate
Controls Nutritionally-Adequate Nutritionally-Adequate

Nutritionally Adequate diet – ad libitum (20% protein; TD 91352, Harlan Teklad, Madison, WI); Low Protein diet – ad libitum (isocaloric, 8% protein; TD 93033, Harlan Teklad, Madison, WI); Energy-Restricted – 70% control animal consumption of Nutritionally Adequate diet.