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Graphical representation of transformation events. Wild type strains UCD 67–385 and CBS-6938 were transformed with the linearized plasmid pBsiWIΔcrtR::hph and circular plasmid pNdeIΔcrtR::hph, respectively. I, II, III: represent crtR exons 1, 2 and 3; P: EF-1α promoter, Term: GPDH transcription terminator; hph: hygromycin B resistance gene; pBS: pBluescript; BsiWI** and NdeI**: modified restriction sites after hygromycin B cassette insertion and arrows represent primer binding sites.
