Table 2.

Plasmids used in this work

Plasmid	Genotype or relevant features	Source of reference
pBluescript SK-	ColE1 ori; AmpR; cloning vector with blue-white selection	Stratagene
pBAD33	p15 ori; CamR; cloning vector	Beckwith Lab.
YIp5	S. cerevisiae/E. coli cloning vector. pMB1 ori; AmpR; TETR; URA3.	ATCC 37061
pET101/D-TOPO	ColE1 ori; AmpR; expression vector	Invitrogene
pDS1B	pBAD33 vector carrying the carotenogenesis genes crtE, crtB, crtI and crtY and crtX from Erwinia uredovora [40].	This work
pPR52.P.44	YIp5 bearing an insert of 12.8 kb that contains the crtR gene from base n° 88 of exon 1.	This work
pCPR1.3	pBluescript SK- bearing the DNA fragment from site BamHI 4,529 to BamHI 9,895 (Figure 1). pCPR1.3 was the crtR deletion platform.	This work
pcXDA.44.29	pBluescript SK- bearing the crtR gene cDNA. The cDNA measures 2,680 bp.	This work
pPR32.AA.51	YIp5 bearing an insert of 4.5 kb that contains the crtR gene up to base n° 87 of exon 1.	This work
pCPRB6	pBluescript SK- bearing an insert of 5.9 kb that contains the complete genomic version of crtR gene. Isolated from a SalI partial genomic library.	This work
pBsiWIΔcrtR::hph	pCPR1.3 BsiWI deletion and hph cassette insertion.	This work
pNdeIΔcrtR::hph	pCPR1.3 NdeI deletion and hph cassette insertion.	This work