Table 3.
Primers used in this work
| Primer | Direction | Sequence 5' to 3' | Target |
| CPR3 | F | CARACTGGKACDGCHGARGATT | crtR gene |
| CPR4 | F | CAAACTGGTACGGCTGAAGATT | crtR gene |
| CPR5 | R | WGGDCCRATCATGAYRACTGG | crtR gene |
| CPR6 | R | AGGTCCAATCATGACGACTGG | crtR gene |
| CPR7 | R | CRGTACCWGGDCCRATCATGA | crtR gene |
| CPR8 | R | CCAGTACCAGGTCCAATCATGA | crtR gene |
| CPR9 | F | GGATCCGCGACATCGAAGAGTATGAC | crtR gene |
| CPR12 | R | GGATCCCTTCCAAGCGAGGTAGTCTT | crtR gene |
| CPR13 | R | AGAAGACTGTGCGATCGTGT | crtR gene |
| CPR31 | R | TCAAGCAATTGGTGTTGGTC | crtR gene |
| CPR56 | R | TCGTTGTGTTGTTGCTATTCG | crtR gene |
| CPR3A | F | TACAACGTCGTCGGTAGACA | crtR gene |
| CPR7A | R | TATTCCACACCGTGGTGTTC | crtR gene |
| CPR3D | F | GGTCTCACTTCTCCAGAGAA | crtR gene |
| CPRfwdC | F | GCACAGGAAGTTGGTTGGAT | crtR gene |
| CPR1exC | F | GCCACACTCTCCGATCTTGT | crtR gene |
| CPREx1fwdb | F | TCAGGACCCTGTACAGTCAGC | crtR gene |
| CPREx1rev | R | TCCCAACAGTCGATCCTTGT | crtR gene |
| Pef | F | GATATCGGCTCATCAGCCGAC | EF-1α Promoter |
| Gpd | R | ATGAGAGATGACGGAGATG | GPDH Terminator |
| HR | R | CTATTCCTTTGCCCTCGGAC | hph gene |
| HF | F | ATGAAAAAGCCTGAACTCACC | hph gene |
| HygSecR | R | GTATTGACCGATTCCTTGCG | hph gene |
| HygSecF | F | TCGCCAACATCTTCTTCTGG | hph gene |
| M13R | R | GGAAACAGCTATGACCATG | pBluescript SK- |
| M13F | F | TGTAAAACGACGGCCAGT | pBluescript SK- |
| ACT3 | F | ACTCCTACGTTGGTGACGAG | Actin gene |
| ACT4 | R | TCAAGTCTCGACCGGCCAAG | Actin gene |
| AST1 | F | GCCACCTACTTTCTCCATATGT | crtS gene |
| AST2 | R | GAGCCATGACGTCCAGAGTA | crtS gene |
F: forward, R: reverse