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(A) p63 immunostaining of a sphere generated by a single sLA7 cell. p63 protein is expressed in self-renewing sLA7 mammospheres (arrows, p63+ cells). Hoechst nuclear dye 33342 (blue). (B) Down-regulation of p63 in sLA7 results in loss of indefinite sphere-regeneration potential. sLA7 cells were cultured with no sense oligos p63 (Sp63), antisense p63 (ASp63), or oligos (control) for 96 h in nonadherent conditions.
