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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2008 Sep 14;15(10):1102–1108. doi: 10.1038/nsmb.1482

Figure 1.

Figure 1

Mal3 promotion of microtubule assembly in vitro. (a) Left, domain structure of Mal3. CH and EB1-like C-terminal motif domains are indicated by green and orange, respectively. Boundaries for each domain are indicated by residue numbers. The amino acid sequence for residues 130-150 is shown below and the three arginine residues are highlighted in red. Right, domain structure for the constructs used in this study. 6×His-tags are highlighted in blue. (b) Microtubule-polymerization assay by 90° light scattering at λ = 350 nm. All assays contained 4 μM S. pombe tubulin (heterodimer concentration). Colored lines indicate the added Mal3 monomer concentration; orange, 0 μM; green, 2 μM; brown, 4 μM; blue, 8 μM.