Fig. 3.

PGRP-SD interacts with both PGRP-SA and GNBP1. (A) rPGRP-SD was immobilized on a CM5 sensor chip, and serial dilutions of rGNBP1 were injected. The RU increased in accordance with the increasing concentration of the injected protein. (B) PGRP-SD stabilized the interaction between GNBP1 and PGRP-SA. rPGRP-SA was immobilized onto the CM5 sensor chip. rGNBP1 and rPGRP-SD were mixed at a 1:1 molar ratio and injected with a serial dilution from 18.5 μM to 0.144 μM. (C) The binding kinetics of all experiments (those described above and all reciprocal orientations) are presented. Ligand refers to the protein coated on the sensor chip. The term analyte is used for the protein injected.