Fig. 3.

FsrA regulation of succinate dehydrogenase expression. (A) Predicted pairing between FsrA and the 5′-leader region of sdhC, the first gene of the sdhCAB operon. (B) Binding of the FsrA to the 5′ leader region of sdhC (200 nM) as detected by EMSA, using radioactively labeled sdhC (200 nM) and cold fsrA transcripts. (C) Level of SdhA protein as detected by proteomics (see Fig. S4 and S5). (D) Growth (OD₆₀₀ after overnight growth at 37°C) in minimal medium containing succinate as carbon source.