Table 2.
PMF in aerobically growing and hypoxic nongrowing Mtb H37Rv
| Drugs (μM) | Δψ, mV | ΔpH, mV | PMF, mV | |
|---|---|---|---|---|
| Aerobic growing mycobacteria | No drug | 66.2 ± 4.4 | 45.3 ± 5.5 | 111.5 ± 9.9 |
| Valinomycin (5) | – | 35.8 ± 2.3 | 35.8 ± 2.3 | |
| Nigericin (5) | 63.1 ± 4.0 | – | 63.1 ± 4.0 | |
| Rifampicin (1) | 62.7 ± 3.2 | 50.6 ± 3.2 | 113.3 ± 6.4 | |
| Hypoxic nongrowing mycobacteria | No drug | 73.1 ± 4.2 | 40.6 ± 9.2 | 113.7 ± 13.4 |
| Valinomycin (5) | – | 31.9 ± 5.3 | 31.9 ± 5.3 | |
| Nigericin (5) | 69.2 ± 10.8 | – | 69.2 ± 10.8 | |
| Rifampicin (1) | 65.1 ± 9.3 | 42.3 ± 8.6 | 107.4 ± 17.9 |
The ΔΨ, ΔpH, and PMF were measured in aerobic growing and hypoxic nonreplicating Mtb maintained in Dubos medium (pH of 6.6). The experiment was carried out three times in triplicate; one of the representative experiment is shown.