Table 1.
Crystallographic data and refinement statistics
| Profilin–actin–VASP202–244 | Gelsolin–actin | Gelsolin–pY53–actin | |
|---|---|---|---|
| Space group | P212121 | C2 | C2 |
| Unit cell a/b/c, Å | 38.04/76.18/180.82 | 178.88/69.17/56.56 | 178.42/69.05/56.54 |
| Unit cell α/β/γ, ° | 90/90/90 | 90/104.36/90 | 90/104.2/90 |
| Resolution, Å | 2.3–50 (2.3–2.38) | 1.6–50 (1.6–1.66) | 1.7–50 (1.7–1.76) |
| Completeness, % | 89.7 (80.9) | 96.8 (78.4) | 94.9(57.9) |
| Multiplicity | 11.0 (11.1) | 7.3 (3.8) | 8.0 (2.1) |
| Rsym, %* | 11.7 (34.9) | 7.1 (52.1) | 8.4 (40.7) |
| I/σ | 20.3 (15.8) | 36.6 (2.1) | 23.1 (2.2) |
| Rfactor, %† | 15.7 | 15.0 | 14.1 |
| Rfree, %‡ | 22.7 | 19.7 | 19.1 |
| rms bonds, Å | 0.015 | 0.011 | 0.012 |
| rms angles, ° | 1.504 | 1.326 | 1.365 |
| B-factor actin/ABP, Å2 | 22.54/20.40 | 24.88/23.21 | 29.40/30.32 |
| B-factor solvent, Å2 | 22.89 | 38.60 | 44.71 |
| Number of aa/waters | 513/318 | 491/461 | 494/460 |
| PDB code | 3CHW | 3CIP | 3CI5 |
Values in parentheses correspond to highest resolution shell.
*Rsym = Σ(I− 〈I〉)/ΣsI; I and 〈I〉, intensity and mean intensity of a reflection.
†Rfactor = Σ|Fo − Fc|/Σ|Fo|; Fo and Fc, observed and calculated structure factors.
‡Rfree; Rfactor of 5% of the reflections that were not used in refinement.