Fig. 2.

Inhibition of changes in mRNA expression of the NMDA receptor subunits by NBQX and CPP. (A) Granule cells were cultured in the serum-containing medium for 24 h. The cultures were continued in the serum-free medium for 96 h after the addition or omission of 100 μM NBQX and 100 μM CPP, as indicated by the shaded bar. The time course of mRNA levels in the presence and absence of antagonists was examined by PCR analysis (n = 5). (B) Cell morphology of granule cells cultured in the serum-free medium at time points 0 and 48 h. Bar, 20 μm. (C) Cultures were treated as described in A, except that the antagonists were added after a 48-h culture period in the serum-free medium (n = 5). Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, antagonist-treated vs. untreated.