Table 2.

Comparison of alignment tensor and sedimentation velocity parameters for tSH2 and tSH2_pm

Protein	Domain	α*, °	β*, °	γ*, °	Aa†	R†	Rdip‡	n§	s20,w	f/f0¶
tSH2	N-SH2	80	65	140	−0.00166	0.39	0.37	29	2.61	1.28
	C-SH2	75	82	131	−0.00161	0.59	0.29	26
tSH2pm	N-SH2	92	49	133	0.00120	0.38	0.34	48	2.28	1.46
	C-SH2	6	31	15	−0.000884	0.44	0.28	50

Alignment was estimated from RDCs of the N-SH2 and C-SH2 domains independently using the program RDCA (22).

*α, β, and γ are Euler angles for the conversion of the alignment tensor frame into the molecular frame.

^†A_a and R are the unitless axial and rhombic components of the alignment tensor.

^‡R^dip=, the quality factor that describes the fit of observed RDCs (D^meas) to RDCs calculated from crystal structure (D^calc).

^§Number of RDC values in the fit.

^¶Friction ratio estimated from s_20,w/s_ma