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. 2008 Aug 12;105(33):11796–11801. doi: 10.1073/pnas.0803078105

Fig. 1.

Fig. 1.

SFEBq culture in growth factor-free medium generates rostral hypothalamic progenitors from ES cells. (A, C, E, G, and I) SFEBq-cultured ES cells using KSR-containing medium, (B, C–E, H, I, K, and N) SFEBq-cultured ES cells using gfCDM. Immunostaining analysis of cryostat sections. (F) Coronal section of mouse rostral diencephalon at E10.5. (J and M) Expression in the rostral–dorsal hypothalamic neuroepithelium (arrowhead) and optic cup (arrow) at E10.5. (L and O) SFEB/DLFA-cultured ES cells for retinal differentiation. The sections were stained with antibodies against Bf1 (A and B), Six3 (D), Rax (G, H, J–O), N-cadherin (A and B), Nestin (J–L), Sox1 (M–O). DAPI for counter staining. (P–X) SFEBq-cultured Rax–EGFP cells are sorted by FACS (P) and analyzed by qPCR on day 7 for the expression of Otx2 (Q), Rax (R), Vax1 (S), Six3 (T), Irx3 (U), En2 (V), Hoxb1 (W), and Hoxb9 (X). Total RNA from E10.5 whole embryos was used as a control.

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