Skip to main content
. 2008 Aug 13;105(33):12057–12062. doi: 10.1073/pnas.0710434105

Fig. 6.

Fig. 6.

Effect of UCP2 on glucagon secretion and pancreatic α-cell mass. Glucagon (A) and insulin (B) secretion from isolated islets in response to changes in glucose concentration (n = 8 for UCP2−/− mice; n = 6 for UCP2+/+ mice). Islets were preincubated in 25 mM glucose for 15 min and then switched to 25 mM glucose (HG) or 2.8 mM glucose (LG) for 1h. For secretion in the presence of arginine (10 mM), islets were incubated with HG (11 mM) or LG (1 mM) for 1h (n = 7 for UCP2−/− mice; n = 6 for UCP2+/+ mice). (C) Glucagon secretion was assessed in α-TC6 cells transfected with UCP2- or control-siRNA in the presence of 25 mM (HG) or 2.8 mM (LG) glucose (n = 3). (D and E) Fasting plasma glucagon (D) (n = 11 mice per group) and intracellular glucagon levels (E) (n = 6 mice per group) of isolated islets are shown. (F) α-cell mass was determined in pancreatic sections from UCP2+/+ (n = 4) and UCP2−/− (n = 5) mice. The glucagon-positive area was calculated and normalized to the islet area. *, P < 0.05; ***, P < 0.001.