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. 2008 Aug 13;105(33):12057–12062. doi: 10.1073/pnas.0710434105

Fig. 7.

Fig. 7.

Effect of UCP2 on islet cell viability under different treatments. (A) Islets from UCP2−/− and UCP2+/+ mice were dispersed (experiment n = 3–6; animal n = 3–8 per group; 623.6 ± 115.8 cells counted per treatment). Insulin negative (i) or positive (ii) cells were treated with medium (10% FBS and 11.5 mM glucose), serum-starved (LSHG) (0.01% FBS and 11.5 mM glucose), cytokines (a combination of IL-1, TNF-α, and IFN-γ at 100 ng of each per milliliter) in culture medium, or PA (0.5 mM) in medium. (B) α-TC6 cells transfected with UCP2- or control-siRNA were seeded at a high or low density and cultured in medium containing 0.05% FBS for three days. Cell viability was determined by using an XTT assay (n = 4). (C) Cell apoptosis was evaluated in α-TC6 cells transfected with UCP2- or control-siRNA by Western blot analysis for activated caspase-3 after treatment as indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001.