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Ubiquitin ligase activity of TRIM2. (A) [35S]Met-labeled TRIM2 autoubiquitination in an in vitro ubiquitination assay in the presence of various ubiquitin-conjugating enzymes (E2). (B) Schematic representation of TRIM2 full-length and mutant constructs. (R, RING finger; B, B box; CC, coiled-coil). (C) In vitro autoubiquitination of full-length and mutant [35S]Met TRIM2 in the absence (−) or presence (+) of UbcH5a.
