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. 1999 Dec;10(12):4201–4215. doi: 10.1091/mbc.10.12.4201

Figure 7.

Figure 7

Arp2p interacts directly with Arp18p. (A) YPB2 carrying pBI-770 ARC18 bait and pBI-771 ARP2 prey plasmids (KGY1897) was grown on medium lacking histidine to demonstrate interaction. KGY1898 and KGY1974 were used as controls. (B) GST-Arc18p fusion protein binds directly to Arp2p. Equal amounts of purified GST (lane 1) or GST-Arc18p (lane 2) bound to glutathione beads were mixed with in vitro translated Arp2p. After extensive washes, the proteins were resolved by SDS-PAGE and autoradiography. Lane 3 represents a sample of in vitro translated Arp2p before the binding reaction. Arrowheads indicate full-length Arp2p. (*) Compression attributable to GST-Arc18p and Arp2p having the same molecular weight.