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Isothermal titration calorimetry data fitted by using a single-site binding model for PixD titrated into PixEHis-6. The fitted parameters are: Ka binding constant = 5.70 ± 0.63 × 106 M−1, n = 2.56 ± 0.027, ΔH = 8.12 ± 0.13 kcal/mol, and ΔS = 0.0586 kcal/(mol·K).
