Fig. 2.

TNFα-mediated NF-κB signaling in skeletal myoblasts depends on cIAP1. (A) C2C12 myoblasts were treated with siRNA for 24 h, treated with TNFα for the indicated times, and immunoblotted for members of the NF-κB signaling pathway. (B) C2C12 myoblasts were treated with siRNA for 24 h, treated with TNFα, and immunoblotted for c-FLIP. (C) C2C12 myoblasts stably expressing an NF-κB luciferase reporter construct were treated with siRNA for 24 h, followed by TNFα for the indicated amounts of time before luciferase activity was measured. Data are expressed mean fold change ± SD, n = 4. (D) Primary skeletal myoblasts were extracted, cultured for 24 h, and treated with TNFα. Protein lysates were collected and immunoblotted for IκBα.