Fig. 5.

Either cIAP1 or 2 is required to protect against TNFα-induced apoptosis. (A) C2C12 myoblasts were treated with siRNA for 24 h, followed by TNFα treatment for 24 h, and cell viability was assessed. Data are expressed as % viability ± SD relative to no TNF treatment controls (set at 100%), n = 4 per condition. (B) C2C12 myoblasts treated with siRNA (24 h) followed by TNFα (24 h) were immunoblotted for markers of caspase activation. (C) C2C12 myoblasts were treated with siRNA and 4 h later infected with adenovirus (Adeno). After 24 h of knockdown, cells were treated with TNFα for 24 h, and cell viability was assessed (as above). (D) C2C12 cells were treated as in C, and protein lysates were immunoblotted for the indicated proteins.