Table 2.

Vesicular aspartate and glutamate transport activity is a special property of sialin

Protein name	Gene name	Uptake, % of control
		Aspartate	Glutamate
Sialin	SLC17A5	100.0 ± 9.2	100.0 ± 9.3
NPT1	SLC17A1	3.9 ± 8.2	0.0 ± 5.1
VGLUT2	SLC17A6	2.7 ± 4.9	116.3 ± 3.1
VGLUT3	SLC17A8	0.0 ± 7.4	60.2 ± 11.2
VNUT	SLC17A9	0.0 ± 0.7	1.1 ± 5.9

Members of the SLC17 family were purified and reconstituted into liposomes. The Δy-dependent uptake of 100 μM aspartate was determined after a 5-min incubation as described in the legend of Fig. 4. The values are expressed as percentages of aspartate uptake (2.9 nmol/min per mg of protein) and glutamate uptake (2.3 nmol/min per mg of protein). The proteoliposomes showed the following transport activities for the authentic substrates: NPT1, Na⁺-dependent phosphate uptake activity, 6.5 nmol/min per mg of protein; VGLUT2, ATP-dependent l-glutamate uptake, 2.8 nmol/min per mg of protein; VGLUT3, ATP-dependent l-glutamate uptake, 1.4 nmol/min per mg of protein; VNUT, ATP uptake, 8.0 nmol/min per mg of protein.