Fig. 1.
The kinase domain of mitochondrial PINK1 is accessible from the cytoplasm. (A) Schematic representation of PINK1 structure. The immunogens used to produce the antibodies (Abs) PINK1175–250 and N4/15 are indicated by the red and blue lines, respectively. (B) SH-SY5Y cells expressing PINK1-IRES-eGFP stably are immunolabeled for a cytosolic protein (α-tubulin), a cytoplasm-facing OMM protein (TOM20), and an IMS protein (ENDOG) (red signals). eGFP is used as a general cell marker (green signals). PINK1 is labeled using PINK1175-250 antibody. (Scale bar, 5 μm.)