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. Author manuscript; available in PMC: 2008 Oct 29.
Published in final edited form as: J Cell Biochem. 2008 Mar 1;103(4):1231–1245. doi: 10.1002/jcb.21503

Fig. 2.

Fig. 2

Effect of Purα on Rev-mediated accumulation of intron-containing unspliced RNA in the cytoplasm of cells. Total (T) and cytoplasmic (C) RNA from U-87MG cells transfected with pIIIAR, pEBV-Purα and pCMV-Rev were analyzed by Northern blot. Probes were RRE (A), CAT (B) and hGAPDH (D). Panel C demonstrates the RNA integrity and the ribosomal RNA bands 28S (5.0 kb) and 18S (1.9 kb) after transfer to a membrane.