Figure 7. RNAPII occupancy on direct target genes of Spt5 in fog^sk8 mutant embryos reveals unexpected complexity and increased 3′ occupancy at the gadd45b locus.

ChIP with RNAPII antibody in WT and fog^sk8 embryos on gadd45b, fos, hsp70, fst, and smo genes show that in fog^sk8 embryos, RNAPII occupancy is significantly increased at 3′ end of gadd45b without increase at 5′ end. Other genes tested showed reduction of RNAPII occupancy at 5′ ends but no change at 3′ ends in fog^sk8 embryos compared to WT. The results shown are the average of at least three independent replicates. Error bars are SEM values.