Skip to main content
. 2008 Nov 14;4(11):e1000202. doi: 10.1371/journal.ppat.1000202

Figure 8. The interaction between MgRac1 and Nox1/Nox2 and superoxide production in MgRac1 mutants.

Figure 8

(A) Yeast two-hybrid assay with MgRac1-CA or MgRac1-DN as the bait and Nox1 or Nox2 as the prey. Yeast transformants grown on the SD-Leu-Trp plates were assayed for β-galactosidase activity. The interaction of pGBKT7-53 and pGADT7-T was used as the positive control. The interaction of BD-MgRac1(CA) and AD (pGADT7) as well as BD (pGBKT7) and AD-Nox1/2 were used as negative controls to rule out self-activation. (B) Bar chart showing mean pixel intensity in hyphal tips and conidia of WT (70-15) and MgRac1 mutants, which quantifies the results in (C) and (D). Increased staining by NBT means reduced pixel intensity. Error bar means standard deviation based on the data of three independent experiments. (C) Detection of superoxide production by 0.6 mM NBT staining in the hyphal tips of WT and MgRac1 mutants. Bar = 10 µm. (D) Detection of superoxide production by 0.3 mM NBT staining in the conidia of WT and MgRac1 mutants. Bar = 10 µm.