Table 1. Phenotypic analysis of MgRac1 mutants.
Strain | Saprophytic growth (mm/day)a | Conidiation (*104)b | Penetration (%)c | Lesions on 5-cm-long rice leaf tipd |
70-15 | 6.18±0.38e | 297.82±16.44 | 70.05±9.03 | 65.45±4.76 |
Guy11 | 5.67±0.62 | 395.83±16.71 | 75.64±7.32 | 72.67±5.11 |
Δ Mgrac1-19 | 5.01±0.17 | 0.07±0.03 | 0 | 0 |
Mgrac1-Com | 5.63±0.26 | 283.32±28.83 | 71.12±6.59 | 61.25±8.18 |
MgRac1-CA | 6.27±0.53 | 154.96±22.74 | 3.66±1.42 | 11.75±3.14 |
MgRac1-DN | 5.28±0.33 | 0.05±0.02 | 0 | 0 |
PCG33 | 5.75±0.02 | 308.9±12.79 | 65.76±6.85 | 63.83±4.65 |
PCA19 | 5.77±0.05 | 265.07±10.43 | 5.14±2.55 | 0 |
chm1 | 3.93±0.66 | 0.55±0.25 | 0 | 0 |
RCC3 f | 5.34±0.73 | 5.35±0.83 | 0 | 0 |
RCC6 f | 5.46±0.04 | 5.4±0.74 | 0 | 0 |
Diameter of hyphal radii at day 8 after incubation on complete medium agar plates at room temperature.
Number of conidia harvested from a 9 cm oatmeal plate at day 10 after incubation at room temperature.
Percentage of penetration over total number of appressoria at 24 h post-inoculation on onion epidermis.
Lesion number 5 days after inoculation.
Data in all columns are the means of three independent experiments with standard deviations.
Two independent transformants expressing constitutively active MgRac1-CA in the chm1 null background.