Table 2. Real-time RT-PCR quantification of MgRac1 expression in M. grisea.
| RNA source | MgRac1 CT a | β-tubulin CT | Normalized MgRac1 level relative to β-tubulinb |
| Wild-type mycelium | 24.43±0.11 | 25.42±0.13 | 1.00 (0.96–1.09)c |
| Wild-type conidium | 21.65±0.06 | 25.63±0.09 | 7.88 (7.84–8.05) |
| Wild-type germ tube | 26.21±0.05 | 25.58±0.10 | 0.33 (0.29–0.35) |
| Wild-type appressorium | 22.64±0.06 | 25.78±0.08 | 4.41 (4.35–4.59) |
a
Cycle number at which the fluorescence crossed the threshold. Mean and standard deviation were calculated with data from three replicates.
b
Relative quantity of MgRac1 at different developmental stages of the wild-type strain 70-15.
c
The mean and range of three replicates.