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. 2008 Nov 14;4(11):e1000205. doi: 10.1371/journal.ppat.1000205

Figure 1. Expression of HTLV-1 receptors, Glut-1 and NP-1, in the thoracic spinal cord from an uninfected individual.

Figure 1

(A,B) Glut-1 immunostaining by immunoperoxydase technique (iPO) (DAB substrate). (C) Dual staining for Glut-1 (iPO, DAB substrate, brown color) and factor VIII (Alcaline phosphatase (AP), FastBlue substrate, blue color). (D,E) NP-1 staining by iPO technique (DAB substrate). Thoracic spinal cord specimens were cut in a cryostat, fixed in methanol and processed for iPO as described in Materials and Methods. (F) Dual staining for NP-1 (iPO, DAB substrate, brown color) and factor VIII (Alcaline phosphatase, FastBlue substrate, blue color). Frozen tissue samples from the thoracic spinal cord were cut on a cryostat at 10 µm, fixed in methanol and processed for immunohistochemistry as described in Materials and Methods. Magnification: (A,D) 5×, (B–E) 50×, (C–F) 100×.