Figure 2. ECRE activity following hyperosmolar or hypoosmolar challenge.

Image series from a single intact mdx fiber either stressed with hypertonic (A) or hypotonic external solution (B). Compared with the initial isotonic condition, osmotic challenge markedly increases apparent SF (C). The response time for the SF increase in hypotonic solution is larger than in hypertonic solution. Apparent SF shows adaptation to ongoing osmotic challenge with a rapid decline before the washout of hyper-/hypotonic medium. Scale bar: 20 µm.