Table 2. Peak SSF, time-to-peak (TTP) and time constant of SSF decline for maintained osmotic challenge, τdec, in wt, mdx and MinD fibers.
strain | Ca2+ | hypertonic | hypotonic | ||||
TTP (s) | Peak SSF (10−3 ECRE/s/100 μm2) | τdec (s) | TTP (s) | Peak SSF (10−3 ECRE/s/100 μm2) | τdec (s) | ||
wt | 2 mM Ca2+ | 30.4±2.9 | 47.9±2.1 | 68.7±7.2 | 52.0±2.5# | 46.1±3.0 | 48.2±5.5# |
mdx | 2 mM Ca2+ | 37.7±1.4* | 117.1±7.0* | 19.3±2.7* | 57.6±0.6* , # | 86.3±4.8* , # | 15.7±1.6* |
0 mM Ca2+ | 10.5±0.5* | 77.6±0.6* | 11.7±1.1* | 14.9±0.6* , # | 81.9±0.6* | 9.1±0.7*,# | |
50 mM Ca2+ | 24.9±4.9 | 106.9±0.7* | 60.0±8.5 | n.a. | n.a. | n.a. | |
MinD | 2 mM Ca2+ | 27.3±3.6 | 45.9±2.4 | 62.0±11.5 | 44.9±3.5# | 45.9±2.1 | 43.6±6.6 |
P<0.05 vs. wt.
P<0.05 hypotonic vs. hypertonic in the same strain.