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. 2008 Nov 5;3(11):e3642. doi: 10.1371/journal.pone.0003642

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Fukada et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Slc39a13 gene is expressed in osteoblasts (1 and 2; red arrow head) in tibia of 4-week-old mice. Regions indicated as 1 and 2 in top are magnified as 400 times. ISH images. B. Slc39a13 mRNA is expressed in proliferative zone of growth plate (left) at 3-week-old tibia. Region indicated as 1 and 2 in top are enlarged at lower panels. PZ: proliferative zone. HZ: hypertrophic zone. C. Slc39a13 gene is expressed in odontoblasts of 10-day-old molar teeth. ISH analysis shows Slc39a13 is expressed in odontoblast (black arrowhead) lining the dentin of crown (1), and in osteoblast (red arrowhead) on the surface of alveolar bone (2). Regions indicated as 1 and 2 in top are magnified as 400 times. od: odontoblasts, ab: alveolar bone. D. Slc39a13 protein expression in dermal fibroblasts. IHC analysis shows Slc39a13 protein is expressed in fibroblasts in dermis of 5-week-old wild-type mice (left upper). Enlarged images of boxed areas are shown in IHC images. Bar indicates 10 µm.