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. 2008 Nov 5;3(11):e3642. doi: 10.1371/journal.pone.0003642

Figure 6. Slc39a13 controls intracellular zinc distribution.

Figure 6

A. Slc39a13 protein locates on perinuclear region in primary osteoblasts (1), chondrocytes (2), pulpal cells (3), and dermal fibroblasts (4). Slc39a13, nuclei, and actin were stained with anti-Slc39a13 specific antibody, DAPI, and phalloidin, respectively. Confocal microscopic images. B. Slc39a13 is localized in Golgi. Confocal microscopic analysis using dermal fibroblasts. Golgi, Slc39a13, nuclei, and actin is stained with anti-GM130 antibody, anti-Slc39a13 specific antibody, DAPI, and phalloidin, respectively. C. Upper: Schematic diagram of EPMA to detect intracellular zinc level. Golgi and nucleus in primary dermal fibroblasts are scanned by electron beam (EB), and characteristic X-ray (CX) for zinc is detected. Lower: Slc39a13 involves in control of intracellular zinc distribution. The ratio of zinc level in Golgi or nucleus versus total Zn in those organelles (n = 11 and 10 for wild-type and Slc39a13-KO dermal fibroblasts, respectively) was obtained by calculating counts per seconds (cps) of CX for zinc generated by EB scanning; Zn level in each organelle (%) = Each organelle Zn (cps)/{nuclear Zn (cps)+Golgi Zn (cps)}×100. Data represents ±S.D.