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. 2008 Nov 5;3(11):e3642. doi: 10.1371/journal.pone.0003642

Figure 7. Slc39a13 is involved in BMP and TGF-β -mediated signaling.

Figure 7

A. Slc39a13 is involved in BMP4 -mediated gene expression. Primary osteoblasts were stimulated with 50 ng/ml of BMP4 for indicated periods, and gene expression including Slc39a13, Msx2, Runx2, and Gapdh was examined by RT-PCR. B. Slc39a13 is involved in TGF-β –mediated gene expression. Primary dermal fibroblasts were incubated with 2.5 ng/ml of TGF-β1 for indicated periods, and gene expression of Slc39a13, Col1a2, Smad7, and Gapdh was examined by RT-PCR. C and D. Ectopic expression of mouse wild-type Slc39a13 (WT), but not of G74D mutant (G74D), rescued BMP4 or TGF-β -induced gene expression in Slc39a13-KO primary osteoblasts (C) or dermal fibroblast (D), respectively, assessed by real-time PCR. Values represent means±S.D. of three separate experiments. E and F. Slc39a13 is involved in BMP/TGF-β –induced nuclear localization of Smad proteins. Primary osteoblasts (E) or dermal fibroblasts (F) were stimulated with either 50 ng/ml of BMP4 for 15 minutes (E, right panels) or 10 ng/ml of TGF-β1 for 30 minutes (F, right panels), respectively, followed by staining for Smad1, Smad2/3, nuclei (DAPI), and actin (Actin). Confocal microscopic images.